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#transposons

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Amelia Cervera 🧬

Relaxed DNA substrate specificity of transposases involved in programmed genome rearrangement.

#Transposons #Tc1Mariner #TelomereBearingElements #Oxytricha_trifallax #Transposase #Genomics #Preprint

biorxiv.org/content/10.1101/20

bioRxiv · Relaxed DNA substrate specificity of transposases involved in programmed genome rearrangementDuring post-zygotic development, the ciliate Oxytricha trifallax undergoes massive programmed genome rearrangement that involves over 225,000 DNA cleavage and joining events. An Oxytricha family of Tc1/mariner transposons, known as Telomere-Bearing Elements (TBEs), encodes a transposase that has been implicated in rearrangement, but its high copy number (>34,000 paralogs) has precluded genetic strategies to investigate its DNA recognition properties directly in Oxytricha. Here, we developed a heterologous strategy to assay TBE transposase expression and activity in E. coli, revealing highly promiscuous DNA cleavage properties. Systematic ChIP-seq experiments allowed us to define the DNA binding specificities of multiple distinct transposase subfamilies, which exhibited a binding and cleavage preference for short, degenerate sequence motifs that resemble features present within the TBE transposon ends. The relaxed sequence preference is striking for autonomous transposases, which typically recognize their end sequences with strict specificity to avoid compromising host fitness. Finally, we developed a custom antibody to investigate TBE transposases in their native environment and found that they precisely localize to the developing nucleus exclusively during the rearrangement process. Collectively, this work establishes a robust heterologous workflow for the biochemical investigation of enzymes that have been repurposed for large-scale genome rearrangements. ### Competing Interest Statement M.W.G.W. is a co-founder of Can9 Bioengineering. S.H.S. is a co-founder and scientific advisor to Dahlia Biosciences, a scientific advisor to CrisprBits and Prime Medicine, and an equity holder in Dahlia Biosciences and CrisprBits.
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Thomas Barrio

Barbara McClintock : Pionnière des Éléments Génétiques Mobiles

🧬🌽 Découvrez Barbara McClintock, généticienne visionnaire ! Sa découverte des éléments génétiques mobiles a révolutionné notre compréhension de l'ADN. De ses travaux sur le maïs au Prix Nobel, plongez dans l'histoire d'une scientifique qui a changé notre vision du génome. #BarbaraMcClintock #Génétique #Transposons #FemmesEnScience Barbara McClintock, généticienne américaine née en 1902, a marqué l'histoire…

homohortus31.wordpress.com/202

Homo Hortus · Barbara McClintock : Pionnière des Éléments Génétiques Mobiles🧬🌽 Découvrez Barbara McClintock, généticienne visionnaire ! Sa découverte des éléments génétiques mobiles a révolutionné notre compréhension de l’ADN. De ses travaux sur le maïs au Prix Nobel…
Public
Amelia Cervera 🧬

Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression.
#SatelliteDNA #Centromers #Chromocenters #piRNA #Transposons #TEs #Drosophila
journals.plos.org/plosbiology/

journals.plos.orgMulti-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repressionPericentromeric heterochromatin clusters into nuclear foci known as chromocenters, the proteome of which remains largely unknown. This study uses quantitative mass spectrometry to characterize the chromocenter-associated proteome in multiple tissues, identifying a link between satellite DNA-binding proteins and transposon repression during embryogenesis.
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